The data set consists of the FlowCAM vignette images and associated files of particles (e.g. protists, zooplankton, inorganic particles) sampled during the K-AXIS (Kerguelen Axis) cruise (Aurora Australis Voyage 3, 2016) from the CTD rosette and underway seawater line. All images selected as non-identified or unwanted particles have been removed from this clean dataset. Calibration and particle library (identified objects) are also included. The "KAXIS_FlowCAM_logsheet.xlsx" file describes all sampling information.

Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.

Sampling sites with a list of activities at each site for the Tangaroa cruise - March to April 2004. Tangaroa Tube Label our use Sample#our use Date(UTC) Time(UTC)Shorthand entry code - ignore Time(UTC)Formatted Use this Long Degdegrees Long Minminutes Long Decdecimaldegrees Lat Degdegrees Lat Minminutes Lat Decdecimaldegrees Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Sea Temp Ice present/absent Lugol's#microscope sample number for phytoplankton ID (Our use only) HPLC Volvolume filtered for HPLC pigment analysis (Our use only) Cocco Volvolume filtered for coccolithophorid counts (Our use only) Cocco tray No.(Our use only) Location:DCM: Deep chlorophyll maximum Thermo: Thermocline

Update - 2013-11-14 - data from the cruise now included in the download file. Two extra excel spreadsheets have been added to the download file - one is a summary file, and the other contains pigment data. Sampling sites with a list of activities at each site for the HIPPIES cruise of the Aurora Australis - December to January 2003-2004. Aurora V4 Tube Labelour use Sample#our use Date(UTC) Time(UTC)Shorthand entry code - ignore Time(UTC)Formatted Use this Long Degdegrees Long Minminutes Long Decdecimaldegrees Lat Degdegrees Lat Minminutes LatDecdecimaldegrees Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Sea Temp Ice present/absent Lugol's#microscope sample number for phytoplankton ID (Our use only) HPLC Volvolume filtered for HPLC pigment analysis (Our use only) Cocco Volvolume filtered for coccolithophorid counts (Our use only) Cocco tray No.(Our use only) Location:DCM: Deep chlorophyll maximum Thermo: Thermocline

Ozone depletion over Antarctica increases UVB irradiances reaching the Earth's surface in the region. Marine microbes, that support the Antarctic food web and play an integral part in carbon cycling, are damaged by UVB. This research determines Antarctic UV climate, biological responses to UV from the molecular to community level, and combines these elements to predict UV-induced changes in Antarctic marine microbiology. A season of field work was undertaken over November and December 1994 based from Davis Station with the intention of making field measurements of ultraviolet radiation in the fast ice environment, as well as some of the lakes in the Vestfold Hills. Instrumentation The instrument for the measurements was a Macam spectral radiometer, owned by Geography and Environmental Studies, University of Tasmania. Field personnel were Dr Kelvin Michael (IASOS) and Mr Michael Wall (Honours student, Geography and Environmental Studies, UTas). The radiometer was equipped with a 25-metre quartz light pipe, with a cosine sensor attachment at the end. To make a measurement of ultraviolet irradiance, the sensor would be oriented so that its sensing surface was horizontal, and it would collect light which was then transmitted along the light pipe to the radiometer - a suitcase-sized unit which ran on battery power in the field. The radiometer was encased in a wooden box lined with polystyrene foam to provide protection from the elements and heat insulation. The radiometer was controlled via a laptop PC and the data were stored on the hard disk of the PC. Measurements Measurements of the attenuation of ultraviolet and visible radiation as a function of wavelength in water were made at the ice edge and lake measurement sites. At the ice edge, the light pipe was spooled over a wheel and lowered to preset depths (typically 1,2,4,8,16 and 32 m below the water surface). On a lake, a 25-cm augur hole was drilled, and the light pipe was lowered by hand to various depths, the exact depths chosen depended on the depth of the lake. Where the lake ice conditions permitted, a frame was lowered through the hole and used to lever the light pipe against the underside of the ice and a measurement of the ultraviolet and visible transmission of the sea ice was collected. In all cases, measurements of the ultraviolet and visible surface irradiance were collected before and/or after the sub-surface measurements. When the sky conditions were sufficiently clear, the direct and diffuse components of the ultraviolet and visible irradiance values were estimated, via the use of a shading apparatus. This would ensure that the radiometer would measure the diffuse component of the radiation field, allowing the direct component to be estimated by subtraction of the diffuse from the global (unshaded) measurement. On some occasions, the upwelling irradiance from the snow or ice surface was also measured, providing information on the spectral albedo of the surface. At each measurement, spectral irradiance values were generally collected for two spectral ranges: UV-B (280 - 400 nm, in 1-nm steps) and visible (400 - 700 nm, in 5-nm steps). In some cases, the wavelength boundaries were different - eg 280 - 350 nm for the UV-B, or 550 - 680 nm in the visible (corresponding to channel 1 of the NOAA AVHRR sensor). The data were stored by the PC as raw data files. The names of these files are automatically defined from the time on the logging PC as 'hhmmss.dti'. Note that the PC was operating on Australian Eastern Summer Time, 4 hours ahead of DLT. These data files were later read into Excel spreadsheets for manipulation. See the linked report for further information. The measurements are all in units of watts per metre squared per nanometre (Wm^-2 nm_-1) The heading UV-B refers to the fact that the data are collected in the ultraviolet part of the spectrum (280 - 400 nm) The heading AVHRR refers to the fact that the data are collected in the visible part of the spectrum (400 - 700 nm) The fields in this dataset are: UV Radiation Wavelength Depth AVHRR

Locations of sampling sites for ASAC project 40 on voyage 3 of the Aurora Australis in the 2006/2007 season. Samples were collected during January and February of 2007. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Locations of sampling sites for ASAC project 40 on voyage 6 of the Aurora Australis in the 1997/1998 season. Samples were collected during March of 1998. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Metadata record for data from ASAC Project 1101 See the link below for public details on this project. ---- Public Summary from Project ---- Most of our knowledge of the Antarctic marine ecosystems comes from summer surveys. There are very few observations of this ecosystem in winter and there is a fundamental lack of knowledge of understanding of even basic questions such as 'what is there?' and 'what's it doing?'. The proposed visit to the sea ice zone in winter is a rare opportunity to conduct observations on phytoplankton, krill, birds, seals and whales, so that we can begin to understand the biological processes that go on in winter. Data for this project were intended to be collected on a 1998 winter voyage of the Aurora Australis, but a fire on board meant that the voyage had to return to port before work could be carried out. Data were then collected the following year during a 1999 winter voyage of the Aurora Australis (IDIOTS), which ran from July to September. Data attached to this metadata record, include protist, bacteria and virus data collected from the Mertz Glacier region. The purpose of this research was to quantify and identify the different assemblages of phytoplankton, change in virus populations and the number of live/dead bacteria between Tasmania and the polynya, within the polynya and sea ice. A variety of methods were used including HPLC, light and fluorescent microscopy and the collection of samples for subsequent analysis upon return to the Antarctic Division. More information is available in the download file. The samples were collected by Rick van den Enden.

Locations of sampling sites for ASAC project 40 on voyage 4 of the Aurora Australis in the 2009/2010 season. Samples were collected during March of 2010. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Project 565: The database provides a list of species of ciliates and testate amoebae (Protozoa: Ciliophora; Testacea) recorded in various edaphic habitats, e.g., mineral soils (fellfield), ornithogenic soils, terrestrial mosses, from ice-free coastal areas and inshore islands in the area of Casey Station, Wilkes Land, coastal continental Antarctica. 26 ciliate (9 first records for continental Antarctica, 1 undescribed) and 5 testacean species (3 new records) were found. Sea ice study (Weddell Sea): The ciliate biodivesity was studied in several types of sea ice (mainly young pancake ice) from the Weddell Sea, Antarctica, in the austral autumn 1992 (March-May) during the cruise ANT X/3 of RV Polarstern. 49 ciliate species were predominantly found in sea ice and 6 spp. in the pelagial; 20 of these were new to science. A word document containing a list of species that were recorded as part of the project is available for download from the provided URL. These data have also been incorporated into the biodiversity database.